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HCV Tridot kit is used for detection of hepatitis C infection by determination of antibodies present in patient's serum. It comprises a small cassette having antigen immobilized porous immunofiltration membrane. This kit is used by adding a drop of sample on cassette and then dispensing protein A conjugate which binds to Fc portion of antibodies in case of positive sample and shows pink colored dots at T1 &/or T2 and control. HCV Tridot is 4th generation rapid & sensitive qualitative method that is capable of detecting NS3 antibodies present at core.